Phylogenomics is a rapidly growing field of study concerned with using genome-wide data—usually in the form of DNA sequence loci—to infer the evolution of genes, genomes, and the Tree of Life. Accordingly, this discipline connects many areas in biology including molecular and genomic evolution, systems biology, molecular systematics, phylogeography, conservation genetics, DNA barcoding, and others. With the advent of Next Generation Sequencing in addition to advances in computer hardware and software over the past decade, researchers can now generate unparalleled phylogenomic datasets that are helping to illuminate many areas in the life sciences. This book is an introduction to the principles and practices of gathering these data. Phylogenomic Data Acquisition: Principles and Practice is intended for a broad cross-section of biologists and anyone else interested in learning how to obtain phylogenomic data using the latest methods.
Table of Contents
Introduction. PHYLOGENOMIC DATA CHARACTERISTICS AND ACQUISITION. Genomes, Molecular Evolution, and Molecular Markers. DNA Extraction. PCR Theory and Methodology. Loci Acquisition. Sanger and Next Generation DNA Sequencing. UNDERSTANDING AND MAKING GENE TREES: THE UNITS OF PHYLOGENOMIC ANALYSIS. Gene Tree Inference. Advanced Gene Tree Inference. PHYLOGENOMIC ANALYSIS: USING GENE TREES TO ANSWER EVOLUTIONARY QUESTIONS. Gene and Genome Evolution. Primer of Coalescent Theory. Species Tree Inference. Phylogeography I. Phylogeography II. DNA Barcoding and Forensics. Future Prospects. References. Glossary. Answers to End-of-chapter Questions.
W. Bryan Jennings is professor and coordinator for the Molecular Laboratory of Biodiversity Research at Universidade Federal do Rio de Janeiro.